Stem cell fate decisions are regulated by the complex interplay of cell-autonomous and non-cell-autonomous (instructive) mechanisms.
These regulatory mechanisms are assembled, at least in part, from molecular components expressed preferentially in stem cells and in stem cell supportive
microenvironments. In order to identify such stem cell or microenvironmental molecular "parts lists" we have focused on the hematopoietic stem cell (HSC)
system in mice and humans. HSC are the best characterized stem cells in the mammal, and have been widely utilized in clinical settings. One approach that
we have utilized to identify molecular "parts lists" is high-throughput DNA sequence analysis of subtracted cDNA libraries constructed from HSC and an HSC
supportive stromal cell line. The sequence sets have been extensively analyzed by computational methods.
This database is intended to be a freely available, and interactive resource for the scientific community. In the near future, SCDb will contain the results
from other efforts to identify stem cell molecular "parts lists".